Biofuel, fuel produced from living organisms (biomass), has recently attracted keen interest for the potential to reduce greenhouse gas emissions as an undepleting alternative for fossil fuel and also as a carbon-neutral (Note 1) fuel, since the amount of CO2 released during combustion of the finished product is offset by the amount of CO2 absorbed by the plants used as raw materials through photosynthesis during the plant growth process. Biofuels include bio-alcohol fuels produced by the fermentation of components extracted from corn, sugar cane and other plants. The Japanese oil industry began introduction of bio-ETBE (Note 2) blend gasoline in 2007. Note 1 Equivalent amounts of CO2 are absorbed and released during the life cycle. Note 2 Abbreviation for ethyl tertiary butyl ether, produced by synthesizing bio-ethanol and isobutylene (oil-based gas). The potential benefits of biofuels produced from algae could be environmentally significant. First, algae directly produces bio-oils through the natural process of photosynthesis, while most conventional biofuels are produced as bio-alcohol by processing raw materials. Since algae absorbs greenhouse gases such as CO2 in the growth process, and are converted into beneficial substances including bio-oils and oxygen, the very process of producing fuel from algae contributes to the reduction of greenhouse gases. Second, bio-oil produced by photosynthetic algae and the resultant biofuel will have molecular structures that are similar to the petroleum and refined products we use today. This helps ensure the fuels are compatible with existing transportation technology and infrastructure. Moreover, algae can be grown using land and water unsuitable for crop plant or food production, unlike some other crop plant-based biofuel sources such as corn or sugar cane, and algae yield more than triple the volume of biofuel on a per-acre basis than any other biofuel sources.